12 – 18 May 2019, Wellcome Genome Campus, UK
Learn the latest experimental approaches for generating genetic mutations in human malaria parasites
This laboratory-based Advanced Course will give participants a working knowledge of, and practical experience in, cutting-edge Plasmodium experimental genetics techniques, from designing gene targeting vectors and creating transgenic parasites to genotyping and phenotyping the resulting strains.
The goal is to benefit the participants’ own research careers by exposing them to state-of-the-art experimental methods, while discussing the advantages and limitations of each approach. The course is targeted to early-career stage malaria scientists who are looking to establish and use Plasmodium transfection technology at their host institutes.
The week-long residential programme will cover experimental approaches to generating genetic mutations in the human malaria parasites Plasmodium falciparum and Plasmodium knowlesi. The course is hands-on and attendees will learn how to generate gene targeting constructs with a particular focus on CRISPR-Cas9 experimental design. Participants will also perform different transfection approaches, undertake practical exercises in genotyping of modified parasites, and both observe and learn advanced molecular phenotyping approaches including drug response assays and flow cytometry. Experimental genetic approaches in other malaria parasites including the rodent malaria model Plasmodium berghei will also be discussed.
The laboratory-based programme will be complemented by specific lectures in cutting-edge areas presented by a panel of distinguished guest speakers.
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