PhD - Royal Veterinary College
A multi-omics approach to improve Eimeria functional genome annotation.
University of London | The Royal Veterinary College | London | United Kingdom
Coccidiosis, an enteric disease caused by Eimeria parasites, is a major threat to food security in poultry production, where it costs ~3 billion USD per year. A group of specialized secretory organelles including the micronemes and rhoptries drive the invasion and intracellular survival of these parasites. Parasite life cycle stages that contain these organelles induce strong protective immunity in the chicken host, so they are of considerable interest for the development of novel vaccines against coccidiosis. However, despite extensive community efforts, there are still 68% of proteins annotated as hypothetical in Eimeria tenella, one of the best-curated Eimeria species, with limited information on function and subcellular localisation. This lack of annotation has restricted the search for additional valid recombinant vaccine candidates.
This project aims to characterise the localisation of organelle proteins using advanced spatial proteomics approaches followed by machine learning based classification solutions. A number of novel protein candidates will be fused with fluorescent reporters to validate localisation and feedback to a bioinformatics pipeline to iteratively improve the prediction and classification accuracy. Data generated will be subsequently integrated with a multi-omics proteogenomic pipeline to further refine gene model predictions in collaboration with EuPathDB team. Functional annotation and domain structure of novel organelle proteins identified will be examined to identify new targets for disease intervention.
Training will be provided and you do not need to be a vet but experience in bioinformatics or genome annotations would be helpful.
Tenure-track positions in Parasitology, McGill University
The Institute of Parasitology at McGill University (http://www.mcgill.ca/parasitology/) seeks to fill two tenure-track positions; the first is at the level of Associate Professor/Full Professor and the second is at level of Assistant Professor. Candidates holding a PhD or equivalent with demonstrated success in research excellence, and potential for high quality teaching in the broad area of Molecular Parasitology or Immunoparasitology are invited to submit their applications. The appointee at the rank of Associate Professor/Full Professor should have a track record commensurate with an appointment as a Tier I Canada Research Chair (http://www.chairs-chaires.gc.ca/home-accueil-eng.aspx). We seek applicants with a history of excellent and impactful publications and successful achievements in securing extramural research funding. Applications are encouraged from scientists working on parasite systems of veterinary and human importance, in particular with research programs focusing on molecular or immunological aspects of parasitology. The appointees are expected to develop a competitive research program supported by external funding and will participate in undergraduate and graduate teaching within the Faculty of Agricultural and Environmental Sciences. The Faculty of Agricultural and Environmental Sciences (http://www.mcgill.ca/macdonald/) and McGill University have a large and dynamic community of researchers focused on parasitic infections, including genomics, proteomics, imaging, drug research and clinical applications provide outstanding opportunities to establish multidisciplinary collaborative projects. Quebec’s prominent and interactive community of parasitologists, who are members of the Centre for Host-Parasite Interactions, provides attractive possibilities to develop new research linkages. McGill University is an English language institution functioning in a bilingual (French, English) environment. Candidates should forward a CV, summary of research plans and the names of three referees by August 1, 2017 to:
Armando Jardim, PhD
Institute of Parasitology
21,111 Lakeshore Road
Ste-Anne-de-Bellevue, Québec, Canada H9X 3V9
Phone (514) 398-7612
Four postdoctoral positions to study the cell biology and drug interactions of the trypanosome trafficking system, Dundee.
Support: The Wellcome Trust
Project director: Mark C. Field
Location: Wellcome Trust Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.
We are seeking four highly motivated postdoctoral researchers with appropriate skills in molecular and cell biology to join our group and our study of the dynamics of the cell surface of trypanosomes. Our major goal is to understand the structure, function and contributions of surface proteins and transport pathways towards antigenic variation, drug sensitivity and pathogenesis. The project is seeking to take studies in this area to the next level, considering the whole cellular context in response to drugs and trafficking, by the application of proteomics, genetic screens and super-resolution microscopy. The School of Life Sciences at Dundee offers exceptional facilities, including world class proteomics, imaging, computation, access to drug screening methodology together with combined expertise in trypanosome research that is excellent. The laboratory is part of the newly formed Wellcome Trust Centre for Anti-Infectives Research. See http://bit.ly/fieldLAB for details.
Successful applicants will pursue a program under the broad title “Dynamics of the cell surface of trypanosomes”, involving predominantly molecular genetics, cell biology, proteomics, biochemistry and structural biology. They will be expected to work independently, to contribute to the teaching/training of other staff/Ph.D. students, to general laboratory duties and preparing and writing manuscripts for publication.
To apply you will have:
• An upper second or first class degree from a first rate UK University of equivalent,
• A recent Ph.D. in a relevant subject, preferably mammalian, yeast or trypanosome cell biology and/or molecular genetics,
• Experience and enthusiasm contributing to molecular cell biology projects,
• Some computer and data processing skills,
• Publications in peer-reviewed journals,
• Experience with cell culture,
• Ability to work independently and as part of a team,
• Excellent communication skills, both written and verbal.
The University of Dundee is committed to equal opportunities and welcomes applications from all sections of the community. The University of Dundee is a Scottish Registered Charity, No. SC015096.
To apply: Please submit a full CV and covering letter directly to email@example.com. Informal inquiries welcome.