PDRA position (12 months) on aquatic symbiosis model systems, based at Edinburgh Napier University and the University of Kent
A 12 month position is available to work with Dr Sonja Rueckert and Dr Anastasios Tsaousis. The PDRA will contribute to the Moore Foundation-funded project developing gregarine Apicomplexa as model systems for aquatic symbiosis.
Further details can be found on this link. Deadline for applications is 25 February 2021
PhD Studentship at Royal Veterinary College, London
Non-RVC Supervisors: Dr. Anastasios Tsaousis
Improvement of Eimeria parasites in vitro culture for host-parasite interactions studies (beginning October 2021)
Seven species of the genus Eimeria (apicomplexan parasites) cause chicken coccidiosis, a costly disease resulting on a reduction of animal performance and welfare. Research into Eimeria species relies heavily on the use of experimental animals due to the lack of in vitro long-term cell culture systems, which is directly related to the complexity of the life cycle of these parasites. However, some in vitro work has allowed the generation of new knowledge about the early stages of Eimeria development. In this project, we propose to conduct a large-scale systematic investigation into the potential use of newly available cell lines and 3D systems for cultivation to improve current in vitro systems for the life-cycle development of Eimeria tenella. In addition, selected cell lines will be used to validate the role of host proteins previously identified as ‘hubs’ in protein interactions networks by a ‘multiomic’ approach. Coding genes for these proteins will be knocked-out (by CRISPR/Cas9 gene editing) or knocked-down (by RNA silencing) to generate important knowledge regarding the host-pathogen interactions in an in vitro system.
More information on the studentship and how to apply can be found here. Deadline for applications is 7 February 2021
Research Associate (21 months) at The University of the Health and Allied Sciences, Ghana
We are seeking to appoint a Research Associate to contribute to a new project on Plasmodium falciparum gametocyte development in the bone marrow of living malaria-infected individuals. We are also accepting Visiting Scientists applications on this project for a period not exceeding 6 months.
Gametocytes of P. falciparum go through several forms of development in different sites of the human body. The earliest forms of gametocytes develop in tissues of the bone marrow, and emerge into circulation when they are mature and are ready to be transmitted to mosquitoes. We have limited understanding of how gametocytes arrive in the human bone marrow, which specific parts of bone marrow tissues they are in, what processes maintain them, and how they are released back into circulation. We are using molecular, immunohistochemical and single cell transcriptomics to perform in-depth investigations in human bone marrow obtained from living individuals in a high malaria transmission area.
This exciting project includes laboratory and field studies based primarily in UHAS, Ho, Ghana, but with the opportunity for experimental work in collaborators’ laboratories at the West African Centre for Cell Biology of Infectious Pathogens, Legon, Accra, Ghana (Prof. Gordon A. Awandare), the Istituto Superiore di Sanità, Rome, Italy (Dr. Pietro Alano), and Molecular Infection Medicine Sweden, Umea, Sweden (Prof. Oliver Billker).
International PhD program in Parasitology as part of the 'ParaFrap' project - 17 positions available
The international PhD program of the French national research project 'ParaFrap' is currently open for applications for 17 PhD positions to be started in September 2021.
PhD Studentship at Aberystwyth University
Helminth parasites are responsible for >55% of livestock diseases representing a major threat to global food security and food borne disease, with control being through anthelmintic drugs due to an absence of vaccines. A major obstacle to improving control options is a lack of understanding of host-parasite interactions. Links between helminth infection, the host and its microbiome are only at a basic level of understanding. Recent work from the Morphew group has suggested that there is a substantial contribution of parasite-mediated changes in the ruminant gut microbiota following investigation into the rumen fluke, Calicophoron daubneyi, within an in vitro rumen model. Furthermore, additional evidence from the Morphew and Huws groups suggests a direct role of rumen fluke-derived extracellular vesicles (EVs) in shaping the microbial communities of the host rumen. Therefore, this project aims to employ a combined meta-omics approach to reveal the interaction between the host rumen microbiome and the rumen fluke and its secreted EVs whilst generating an understanding of rumen functionality following exposure to the parasite or parasite derived EVs. This project will aim to 1) Confirm EV release from C. daubneyi into rumen fluid 2) assess any antimicrobial effect of C. daubneyi EVs on bacteria 3) Employ meta-omic techniques to assess the effect of C. daubneyi or C. daubneyi derived EVs on the rumen microbiome using an in vitro fermentation model and 4) Confirm in vitro findings in vivo. The discovery of the role rumen fluke infection has upon the ruminant microbiome will provide insights into any knock on effect on rumen function and thus animal productivity.
This is a CASE project with in-kind support from Ridgeway Research Ltd, a UK-based veterinary serviced firm who will provide samples, as well as placement and diagnostics training for the successful student.
For more information and how to apply, see this link. Deadline for applications: 8 February 2021